Journal: Scientific Reports
Article Title: MT1G promotes iron autophagy and inhibits the function of gastric cancer cell lines by intervening in GPX4/SQSTM1
doi: 10.1038/s41598-024-80160-4
Figure Lengend Snippet: A correlation between the effect of MT1G on GC cell lines and the occurrence of ferroptosis. ( a ) MDA expression in different GC cell lines. ( b ) The expression of SOD in different GC cell line. ( c ) The expression of GSH in different GC cell lines. ( d ) The expression of ferroptosis related proteins in different GC cell lines. ( e ) The expression of GPX4 protein in different GC cell lines. ( f ) The expression of FSP1 protein in different GC cell lines. ( g ) Expression of SLC7A11 protein in different GC cell lines. ( h ) The expression of DHODH protein in different GC cell lines. ( i ) Fluorescence intensity of Fe 2+ content. Scale bar = 50 μm. * p < 0.05; ** p < 0.01; *** p < 0.001.The original images for western blot analysis were shown in “Supplementary information”.
Article Snippet: After 2 h of closure with skimmed milk in TBST, the membranes were analyzed using SQSTM1 (Immununoway: YT7058), LC3B (Immununoway: YN5524), EGLN2 (Boster: BM3977), GAPDH (Boster: BA2913), GPX4 (Boster: BM5231), FSP1 (Proteintech: #20,886–1-AP), DHODH (Boster: M04035-1) and antibodies to SLC7A11 (Boster: BM5318) were incubated overnight at 4 °C.
Techniques: Expressing, Fluorescence, Western Blot